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Anti-Hu CD132 Purified

CD132 / common gamma chain is an essential component of receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, and it is critical for development of the immune system. Its mutation causes X-linked severe combined immunodeficiency disease (XSCID) . CD132 is expressed on lymphocytes, NK cells, monocytes, and granulocytes. Through its cytoplasmic part which containsfour tyrosines and an SH2 domain, CD132 transcuces signal to downstream JAK/STAT pathway.

Product Specifications

Certification

RUO

Reactivity

Human, Dog

Immunogen

Human CD132-transfected cell line

Target Antigen

CD132

Clone

TUGh4

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Corrigall VM, Arastu M, Khan S, Shah C, Fife M, Smeets T, Tak PP, Panayi GS: Functional IL-2 receptor beta (CD122) and gamma (CD132) chains are expressed by fibroblast-like synoviocytes: activation by IL-2 stimulates monocyte chemoattractant protein-1 production. J Immunol. 2001 Mar 15;166 (6) :4141-7., URL: https://pubmed.ncbi.nlm.nih.gov/11238664/, *Brisslert M, Bokarewa M, Larsson P, Wing K, Collins LV, Tarkowski A: Phenotypic and functional characterization of human CD25+ B cells. Immunology. 2006 Apr;117 (4) :548-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/16556269 , *Amu S, Brisslert M: Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin Dev Immunol. 2011;2011:481948., URL: http://www.ncbi.nlm.nih.gov/pubmed/21941578 , *Fiorito S, Magrini L, Adrey J, Mailhé D, Brouty-Boyé D: Inflammatory status and cartilage regenerative potential of synovial fibroblasts from patients with osteoarthritis and chondropathy. Rheumatology (Oxford) . 2005 Feb;44 (2) :164-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/15479753 , *Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71 (4) :329-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/20074604 , *Itano M, Tsuchiya S, Morita S, Fujie H, Ishii N, Yanagisawa T, Ohashi Y, Minegishi M, Sugamura K, Konno T: IL-2 receptor gamma chain expression on CD34 positive hematopoietic progenitor cells from bone marrow and cord blood. Tohoku J Exp Med. 1996 Apr;178 (4) :389-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/8804156

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody TUGh4 recognizes an extracellular epitope of CD132 (the common gamma chain), a 65-70 kDa type I transmembrane glycoprotein broadly expressed by most leukocytes.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Isotype

Rat IgG2b kappa

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