Anti-Ag85b (M. tuberculosis) Purified
The Mycobacterium tuberculosis antigen Ag85b, also known as FbpB secreted antigen, 85-B FbpB (85B), antigen 85 complex B, mycolyl transferase 85B, fibronectin-binding protein B, or extracellular alpha-antigen, facilitates Mycobacterium attachment to host cells via fibronectin. It is also involved in maintaining cell wall integrity through the transfer of mycolyic acids (diacylglycerol acyltransferase/mycolyltransferase activity) .
Product Specifications
Certification
RUO
Reactivity
Mycobacterium tuberculosis
Immunogen
Recombinant Mycobacterium tuberculosis Ag85b produced in E. coli
Target Antigen
Ag85b (M. tuberculosis)
Clone
PAb (189)
Applications
WB, ELISA
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Other References
*Backus KM, Dolan MA, Barry CS, Joe M, McPhie P, Boshoff HI, Lowary TL, Davis BG, Barry CE: The three Mycobacterium tuberculosis antigen 85 isoforms have unique substrates and activities determined by non-active site regions. J Biol Chem. 2014 Sep 5;289 (36) :25041-53., URL: http://www.ncbi.nlm.nih.gov/pubmed/25028517
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The rabbit polyclonal antibody to Ag85b reacts with the Mycobacterium tuberculosis protein Ag85b (FbpB), which is a diacylglycerol acyltransferase/mycolyltransferase.
Isotype
Rabbit polyclonal
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