Anti-Hu CD22 Purified

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Whole hairy cell leukemia cells and membrane preparation

Target Antigen

CD22

Clone

S-HCL-1

Applications

FC (QC tested), WB

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ: CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti-CD22 antibodies to peripheral blood dendritic cells. Cytometry B Clin Cytom. 2009 Jul;76 (4) :237-48, URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=Reineks+EZ%2C+Osei+ES%2C+Rosenberg+A , *James SE, Greenberg PD, Jensen MC, Lin Y, Wang J, Till BG, Raubitschek AA, Forman SJ, Press OW: Antigen sensitivity of CD22-specific chimeric TCR is modulated by target epitope distance from the cell membrane. J Immunol. 2008 May 15;180 (10) :7028-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/18453625 , *Huang J, Fan G, Zhong Y, Gatter K, Braziel R, Gross G, Bakke A: Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry. Am J Clin Pathol. 2005 Jun;123 (6) :826-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/15899772 , *Foon KA, Todd RF 3rd: Immunologic classification of leukemia and lymphoma. Blood. 1986 Jul;68 (1) :1-31., URL: http://www.ncbi.nlm.nih.gov/pubmed/2941082

Other References

*Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7, URL: https://www.ncbi.nlm.nih.gov/pubmed/18067554?ordin, *Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27 (16) :5699-710., URL: https://www.ncbi.nlm.nih.gov/pubmed/17562860?ordin, *Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/16227086?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Applications Notes

Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions.