Anti-Cyclin D1 Purified
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Product Specifications
Certification
RUO
Negative Species
Cat,Sheep
Immunogen
Recombinant human cyclin D1 (amino acids 1-295)
Target Antigen
Cyclin D1
Clone
DCS-6
Applications
FC-IC, IP, WB, IHC-P, IHC-F, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
Other References
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
Species
Rat, Mouse, Non-human primates, Human
CAS Number
9007-83-4
Isotype
Mouse IgG2a
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