Anti-Hu CD165 Purified
CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
CD165 purified from human Molt-4 cell line
Target Antigen
CD165
Clone
SN2
Applications
FC (QC tested), IP, WB, IHC-F, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Seon BK: Specific killing of human T-leukemia cells by immunotoxins prepared with ricin A chain and monoclonal anti-human T-cell leukemia antibodies. Cancer Res. 1984 Jan;44 (1) :259-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/6606488 , *Seon BK, Negoro S, Barcos MP, Tebbi CK, Chervinsky D, Fukukawa T: Monoclonal antibody SN2 defining a human T cell leukemia-associated cell surface glycoprotein. J Immunol. 1984 Apr;132 (4) :2089-95., URL: http://www.ncbi.nlm.nih.gov/pubmed/6607954
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.
Applications Notes
Immunohistochemistry (frozen sections) : Application note: acetone fixation. Flow cytometry: Recommended dilution: 1-4 µg/ml.Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Isotype
Mouse IgG1 kappa
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