Anti-Hu CD170 Purified

CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs) . CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a) . Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human

Immunogen

Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1

Target Antigen

CD170

Clone

1A5

Applications

FC (QC tested), IP, IHC-F, ICC, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Avril T, Freeman SD, Attrill H, Clarke RG, Crocker PR: Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem. 2005 May 20;280 (20) :19843-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=avril+t+freeman+sd, *Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS: Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy. 2006 Jun;61 (6) :769-76., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=yokoi+h+myers+a , *Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M: Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 2006 Oct;20 (12) :1964-73., URL: http://www.ncbi.nlm.nih.gov/pubmed/17012248 , *Schuster P, Donhauser N, Pritschet K, Ries M, Haupt S, Kittan NA, Korn K, Schmidt B: Co-ordinated regulation of plasmacytoid dendritic cell surface receptors upon stimulation with herpes simplex virus type 1. Immunology. 2010 Feb;129 (2) :234-47., URL: http://www.ncbi.nlm.nih.gov/pubmed/19824924

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.