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Anti-Hu CD151 Purified

CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3 / beta 1 (CD49c / CD29), alpha 5 / beta 1 (CD49e / CD29), and alpha 6 / beta 4 (CD49f / CD104) . CD151 appears to be involved in cell adhesion and migration, including metastasis.

Product Specifications

Certification

RUO

Immunogen

Human epidermoid carcinoma cell line Hep-3

Target Antigen

CD151

Clone

50-6

Applications

IHC-F, WB, FC (QC tested), ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Sadej R, Grudowska A, Turczyk L, Kordek R, Romanska HM: CD151 in cancer progression and metastasis: a complex scenario. Lab Invest. 2014 Jan;94 (1) :41-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/24247563, *Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, Anstee DJ: Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One. 2013 May 21;8 (5) :e62654., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=spring+fa+griffiths+re Testa JE Brooks PC Lin JM Quigley JP: Eukaryotic expression cloning with an antimetastatic monoclonal antibody identifies a tetraspanin (PETA-3/CD151) as an effector of human tumor cell

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.

Applications Notes

Flow cytometry: Recommended dilution: 2-4 μg/ml.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

Curated Selection

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