Anti-Hu CD15 Purified

CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le (x) antigen exhibit strong self-aggregation, based on calcium-dependent Le (x) -Le (x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le (x) and its isomer sialyl-Le (a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

U937 histiocytic lymphoma cells

Target Antigen

CD15

Clone

MMA

Applications

FC (QC tested), IHC-P, IHC-F, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Chaubey S, Wolfe JH: Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage. Stem Cells Transl Med. 2013 Jun;2 (6) :444-54., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=chaubey+s+wolfe+jh , *Mao XG, Zhang X, Xue XY, Guo G, Wang P, Zhang W, Fei Z, Zhen HN, You SW, Yang H: Brain Tumor Stem-Like Cells Identified by Neural Stem Cell Marker CD15. Transl Oncol. 2009 Dec;2 (4) :247-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/19956386 , *Capela A, Temple S: LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1. Dev Biol. 2006 Mar 15;291 (2) :300-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16458284 , *Pellegrini W, Bresciani G, De Zorzi A, Marocolo D, Ungari M, Facchetti F: MMA monoclonal antibody is a superior anti-CD15 reagent for the diagnosis of classical Hodgkin's lymphoma? Haematologica. 2007 May;92 (5) :708-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pellegrini+w+bresciani+g, *Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94 (1) :23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353, *Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul;94 (4) :637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839

Other References

*Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39 (1-2) :185-94., URL: https://pubmed.ncbi.nlm.nih.gov/10975398/, *Hakomori S: Le (X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24 (11) :771-6., URL: https://pubmed.ncbi.nlm.nih.gov/1362193/, *Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le (X) but is inhibited by sLe (X) . Biochem J. 2007 Sep 1;406 (2) :333-41., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody MMA reacts with an extracellular epitope of CD15, a cell membrane 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This antibody is a superior reagent for identifying of Hodgkin´s lymphoma.