Anti-Hu CD49c Purified
CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen) . It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Human SSC-9 cell line (squamous cell carcinoma)
Target Antigen
CD49c
Clone
ASC-1
Applications
FC (QC tested), IP, WB, IHC-F
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS: Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. Am J Pathol. 1996 May;148 (5) :1445-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=wilke+ms+letourneau+pc , *Pattaramalai S, Skubitz KM, Skubitz AP: A novel recognition site on laminin for the alpha 3 beta 1 integrin. Exp Cell Res. 1996 Feb 1;222 (2) :281-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pattaramalai+s+skubitz+km
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.
Applications Notes
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Isotype
Mouse IgG1 kappa
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