Anti-Cytokeratin 19 Purified
Selected Variant
11-119-C100
Size: 0.1 mg
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Mammary organoids.
Target Antigen
Cytokeratin 19
Clone
BA-17
Applications
WB, IHC-P, IP, ICC, FC-IC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Bartek J, Taylor-Papadimitriou J, Miller N, Millis R: Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours. Int J Cancer. 1985 Sep 15;36 (3) :299-306., URL: https://www.ncbi.nlm.nih.gov/pubmed/2411673?dopt=A, *Bartek J, Durban EM, Hallowes RC, Taylor-Papadimitriou J: A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J Cell Sci. 1985 Apr;75:17-33. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2413060?dopt=A, *Lewis BC, Klimstra DS, Varmus HE: The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer. Genes Dev. 2003 Dec 15;17 (24) :3127-38. Epub 2003 Dec 17., URL: http://genesdev.cshlp.org/content/17/24/3127.full
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody BA-17 reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Applications Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 5-10 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
Isotype
Mouse IgG1 kappa
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