Anti-Cytokeratin 7/17 Purified

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Product Specifications

Certification

RUO

Negative Species

Rabbit,Mouse,Rat

Reactivity

Pig, Human, Cow

Immunogen

Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Target Antigen

Cytokeratin 7/17

Clone

C-46

Applications

IP, WB, IHC-P, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Vojtesek B, Staskova Z, Nenutil R, Bartkova J, Kovarik J, Rejthar A, Bartek J: A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 1990;37 (3) :333-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/1695327?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa) . Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells.