Anti-Cytokeratin 7/17 Purified
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Product Specifications
Certification
RUO
Negative Species
Rabbit,Mouse,Rat
Reactivity
Pig, Human, Cow
Immunogen
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Target Antigen
Cytokeratin 7/17
Clone
C-46
Applications
IP, WB, IHC-P, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Vojtesek B, Staskova Z, Nenutil R, Bartkova J, Kovarik J, Rejthar A, Bartek J: A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 1990;37 (3) :333-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/1695327?dopt=A
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa) . Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells.
Isotype
Mouse IgG1
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