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Anti-Cytokeratins Purified

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Product Specifications

Certification

RUO

Reactivity

Mammalian

Immunogen

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Target Antigen

Cytokeratins

Clone

C-11

Applications

FC-IC, WB, IP, IHC-P, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101 (2) :115-26., URL: https://pubmed.ncbi.nlm.nih.gov/9821208/, *Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68 (6) :2572-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/16316332?dopt=, *Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha) . 1989;35 (6) :373-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/2483834, *Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2463228?dopt=A, *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Applications Notes

Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.

Isotype

Mouse IgG1

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