Anti-Cytokeratin 18 Purified

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins) .

Product Specifications

Certification

RUO

Negative Species

Dog,Mouse,Rat,Pig,Hamster,Sheep,Cow

Reactivity

Human

Immunogen

Human breast carcinoma cell line PMC-42.

Target Antigen

Cytokeratin 18

Clone

DC-10

Applications

FC-IC, IP, WB, IHC-P, ICC, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7 (5) :495-504., URL: https://www.ncbi.nlm.nih.gov/pubmed/2461901?dopt=A, *Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/2463228?dopt=A, *Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha) . 1989;35 (6) :373-82. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2483834?dopt=A

Other References

*Čáp J, Čermáň J, Němeček S, Marková M, Haná V, Frysák Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10 (4) :444-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12852883?dopt=, *Kovařík J. et al., J. Tumor Marker Oncol. 5, 219 (1990) ., URL: , *Taylor-Papadimitriou J, Stampfer M, Bártek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 (Pt 3) :403-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/2483723?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody DC-10 reacts with cytokeratin 18 (45 kDa) . Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.

Applications Notes

Western blotting: Recommended dilution: 1-2 μg/ml. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100) . Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.