Anti-Cytokeratin 8 Purified
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Product Specifications
Certification
RUO
Negative Species
Mouse,Rat,Xenopus,Hamster,Chicken
Reactivity
Cow, Human, Rabbit, Pig, Sheep
Immunogen
Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
Target Antigen
Cytokeratin 8
Clone
C-43
Applications
IP, WB, IHC-P, ICC, FC-IC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990) ., URL: , *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A, *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen) . Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: prostate. Flow cytometry: Recommended dilution: 0.5-4 μg/ml. Intracellular staining.
Isotype
Mouse IgG1
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