Anti-Cytokeratin 8 Purified

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Product Specifications

Certification

RUO

Negative Species

Mouse,Rat,Xenopus,Hamster,Chicken

Reactivity

Cow, Human, Rabbit, Pig, Sheep

Immunogen

Cytoskeleton preparation from HeLa human cervix carcinoma cell line.

Target Antigen

Cytokeratin 8

Clone

C-43

Applications

IP, WB, IHC-P, ICC, FC-IC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990) ., URL: , *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A, *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen) . Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: prostate. Flow cytometry: Recommended dilution: 0.5-4 μg/ml. Intracellular staining.