Anti-Cytokeratin 5/8 Purified

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Product Specifications

Certification

RUO

Negative Species

Xenopus,Chicken

Reactivity

Rat, Hamster, Pig, Sheep, Dog, Cow, Human, Mouse

Immunogen

Cytoskeleton preparation from HeLa cells.

Target Antigen

Cytokeratin 5/8

Clone

C-50

Applications

IHC-P, IP, WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody C-50 reacts with cytokeratin peptides 5 and 8 (58 and 53 kDa) . Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive control: human prostate.Western blotting: Recommended dilution: 1-2 μg/ml.