Anti-Cytokeratin 5/8 Purified
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Product Specifications
Certification
RUO
Negative Species
Xenopus,Chicken
Reactivity
Rat, Hamster, Pig, Sheep, Dog, Cow, Human, Mouse
Immunogen
Cytoskeleton preparation from HeLa cells.
Target Antigen
Cytokeratin 5/8
Clone
C-50
Applications
IHC-P, IP, WB, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody C-50 reacts with cytokeratin peptides 5 and 8 (58 and 53 kDa) . Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive control: human prostate.Western blotting: Recommended dilution: 1-2 μg/ml.
Isotype
Mouse IgG1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC401429-100 1x 100 µL
E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC681073-500 1x 500 µL
HLA-Aw32 & HLA-A25 (MHC I) (CATA-1), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680633-100 1x 100 µL
Von Willebrand Factor (3E2D10), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC041037-500 1x 500 µL
CD44 Standard (DF1485), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC940977-100 1x 100 µL
TGF-beta (1D11.16.8), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details