Anti-Ms CD90.2 Purified Azide Free

CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.

Product Specifications

Certification

RUO

Negative Species

Rat,Human

Reactivity

Mouse

Immunogen

Thymocytes from C3H/Di mice (Thy-1.2 positive)

Target Antigen

CD90.2

Clone

1aG4/C5

Applications

FC (QC tested), WB, ICC, FUNC

Concentration

1 mg/mL

Format

Purified Azide Free

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Draberova L, Draber P: Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells. Proc Natl Acad Sci U S A. 1993 Apr 15;90 (8) :3611-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/7682713?dopt=A, *Bradley JE, Ramirez G, Hagood JS: Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 2009 May-Jun;35 (3) :258-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/19422052, *Heneberg P, Lebduška P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36 (10) :2795-806., URL: https://www.ncbi.nlm.nih.gov/pubmed/17013982?dopt=, *Cikán J, Dráber P, Vojtíšková M: Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region. Folia Biol (Praha) . 1982;28 (6) :377-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/6186535?dopt=A, *Dráber P, Cikán J, Vojtíšková M: Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies. J Immunogenet. 1980 Dec;7 (6) :455-74. , URL: https://www.ncbi.nlm.nih.gov/pubmed/6787139?dopt=A

Other References

*Rege TA, Hagood JS: Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses. Biochim Biophys Acta. 2006 Oct;1763 (10) :991-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/16996153, , *Haeryfar SM, Hoskin DW: Thy-1: more than a mouse pan-T cell marker. J Immunol. 2004 Sep 15;173 (6) :3581-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/15356100, , *Rege TA, Hagood JS: Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 2006 Jun;20 (8) :1045-54., URL: http://www.ncbi.nlm.nih.gov/pubmed/16770003

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90) ; it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily) ; in mouse it is abundant on thymocytes and peripheral T lymphocytes.

Applications Notes

Functional application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Immunoprecipitation: The antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase. Flow cytometry: Recommended dilution: 1 μg/ml.