Anti-Hu CD54 Purified Azide Free

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1) . The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Raji cells and spleen cells fused with NS1 cells

Target Antigen

CD54

Clone

1H4

Applications

FC (QC tested), WB, IHC-P, IHC-F, ICC

Concentration

1 mg/mL

Format

Purified Azide Free

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar;85 (Pt 3) :731-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/14993659?dopt=

Other References

*Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85 (9) :3095-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3362863?dopt=A, *Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73 (7) :1896-903., URL: https://www.ncbi.nlm.nih.gov/pubmed/2469503?dopt=A, *Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2;346 (6283) :425-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/1974032?dopt=A, *Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 10;68 (1) :63-9. Erratum in: Cell 1992 Mar 6;68 (5) :following 994., URL: https://www.ncbi.nlm.nih.gov/pubmed/1346257?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Applications Notes

Immunohistochemistry (frozen sections) : Recommended dilution: 5-10 μg/ml. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.