Anti-Hu CD59 Purified Azide Free

CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC) . It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Product Specifications

Certification

RUO

Reactivity

Pig, Human

Immunogen

Human peripheral blood lymphocytes

Target Antigen

CD59

Clone

MEM-129

Applications

FC (QC tested), FUNC

Concentration

1 mg/mL

Format

Purified Azide Free

Buffer

Tris buffered saline (TBS), pH 8.0

References & Citations

*Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272 (31) :19242-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9235917, *Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85 (9) :2402-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/7537112

Other References

*Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173 (6) :3684-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/15356114?ordin, *Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153 (6) :2444-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/7521361?ordina, *Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83 (2) :251-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1704296?ordina, *Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71 (1) :1-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1698710?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-129 reacts with CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI) -anchored extracellular glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissus.