Anti-Hu CD11b Purified Azide Free

*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19 (5) :675-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/17446208?itool=, *Hasan S, Osickova A, Bumba L, Novák P, Sebo P, Osicka R: Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding. FEBS Lett. 2015 Jan 30;589 (3) :374-9., URL: https://pubmed.ncbi.nlm.nih.gov/25554420/

*Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95 (3) :911-20., URL: https://pubmed.ncbi.nlm.nih.gov/10648403/, *Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119 (3-4) :287-98., URL: https://pubmed.ncbi.nlm.nih.gov/17628696/, *Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas) . 2007;43 (8) :597-606., URL: https://pubmed.ncbi.nlm.nih.gov/17895634/

The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes. Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants. Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns) . The final immunosorbent should contain 1-5 mg of antibody/1 mL of sorbent gel (e.g. agarose beads) . It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.