LIN28A (phospho-Ser200) rabbit pAb

This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015],

Product Specifications

Background

Product Name Alternative

Protein lin-28 homolog A (Lin-28A) (Zinc finger CCHC domain-containing protein 1)

UniProt

Q9H9Z2

Swiss Prot

Q9H9Z2

Reactivity

Human; Rat; Mouse

Immunogen

Synthesized phosho peptide around human LIN28A (Ser200)

Target

LIN28A

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300

Buffer

-20°C/1 year

Molecular Weight

23kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

23kD

Fragment

IgG

Subcellular Location

Cytoplasm . Rough endoplasmic reticulum . Cytoplasm, P-body . Cytoplasm, Stress granule . Nucleus, nucleolus . Predominantly cytoplasmic (PubMed:22118463) . In the cytoplasm, localizes to peri-endoplasmic reticulum regions and detected in the microsomal fraction derived from rough endoplasmic reticulum (RER) following subcellular fractionation. May be bound to the cytosolic surface of RER on which ER-associated mRNAs are translated (By similarity) . Shuttle from the nucleus to the cytoplasm requires RNA-binding (PubMed:17617744) . Nucleolar localization is observed in 10-15% of the nuclei in differentiated myotubes (By similarity) . .