Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

<strong>Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.</strong>_x000D_ <strong>Catalog number:</strong> B2019702_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 250 M IU_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> SCDase_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Han YB, Wu L, Rich JR, Huang FT, Withers SG, Feng Y, Yang GY. Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids Appl Microbiol Biotechnol. 2015 Aug;99(16):6715-26._x000D_ 2: Kuchar L, Rotková J, Asfaw B, Lenfeld J, Horák D, Korecká L, Bílková Z, Ledvinová J. Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry Rapid Commun Mass Spectrom. 2010 Aug 30;24(16):2393-9._x000D_ 3: Ito M, Kurita T, Kita K. A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms J Biol Chem. 1995 Oct 13;270(41):24370-4._x000D_ 4: Okino N, Ito M. Hydrolysis of glycosphingolipids by a sphingolipid ceramide N-deacylase (SCDase) 2021 Sep 28 [updated 2022 Mar 18]. In: Nishihara S, Angata K, Aoki-Kinoshita KF, Hirabayashi J, editors. Glycoscience Protocols (GlycoPODv2) [Internet]. Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021–._x000D_ 5: Tirodkar TS, Voelkel-Johnson C. Sphingolipids in apoptosis Exp Oncol. 2012 Oct;34(3):231-42._x000D_ 6: Mitsutake S, Kita K, Okino N, Ito M. [14C]ceramide synthesis by sphingolipid ceramide N-deacylase: new assay for ceramidase activity detection Anal Biochem. 1997 Apr 5;247(1):52-7._x000D_ 7: Cho KW. Bioluminescent assay for sphingolipid ceramide N-deacylase using Vibrio harveyi dark mutant M-17 J Microbiol. 2008 Oct;46(5):585-9._x000D_ 8: Furusato M, Sueyoshi N, Mitsutake S, Sakaguchi K, Kita K, Okino N, Ichinose S, Omori A, Ito M. Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8 J Biol Chem. 2002 May 10;277(19):17300-7._x000D_ 9: Nakagawa T, Tani M, Kita K, Ito M. Preparation of fluorescence-labeled GM1 and sphingomyelin by the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase as substrates for assay of sphingolipid-degrading enzymes and for detection of sphingolipid-binding proteins J Biochem. 1999 Sep;126(3):604-11._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/26130766">10: Huang FT, Han YB, Feng Y, Yang GY. A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production J Lipid Res. 2015 Sep;56(9):1836-42. </a>_x000D_ _x000D_ <strong>Products Related to Sphingolipid Ceramide N-Deacylase, Pseudomonas sp. can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>