Biotinylated Human HLA-A*11:01&B2M&MAGE-A1 (SLFRAVITK) Complex Protein (Monomer, MALS verified)

MAGE-A1 is a cancer testis antigen that is expressed in a variety of malignancies, including multiple myeloma, melanoma, lung, breast, colon, and ovarian cancer. The Human HLA-A*0201 MAGE-A1 (SLFRAVITK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and SLFRAVITK peptide of the MAGE-A1 (96-104) .

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*11:01&B2M&MAGE-A1 (SLFRAVITK) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A1 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & SLFRAVITK

Expression Region

Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & SLFRAVITK peptide

Host

HEK293

Target

HLA-A*1101 & B2M & MAGE-A1 (SLFRAVITK)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*11:01&B2M&MAGE-A1 (SLFRAVITK) Complex Protein (Cat. No. HLM-H82Eh) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).