Phi29 DNA polymerase (10 U/µL)

Phi29 DNA polymerase, derived from Bacillus subtilis phage, has been genetically engineered to exhibit excellent strand displacement and continuous synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb. Additionally, it has strong 3'→5' exonuclease proofreading activity, thus it is recommended to modify the 3' end of primers in the system to prevent degradation. It is commonly used for in vitro plasmid synthesis and whole genome synthesis.