Anti-KDEL Antibody

Mouse Monoclonal Antibody specific to KDEL Antibody

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-KDEL Antibody Mouse Monoclonal Antibody

Host

Mouse

Reactivity

All

Immunogen

Peptide corresponding to aa 649-654 of rat Grp78 conjugated to KLH.

Target

KDEL

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ICC, ELISA

Field of Research

Purification Method

Purified by affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: Use at 4ug/ml. Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants. Immunohistochemistry: Use at 5- 10ug/ml. These are recommended concentrations. End users should determine optimal concentrations for their applications.

Storage Conditions

Stable for at least one year at or below -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

KDEL Antibody

Formulation

PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.2

Target Background

The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor which is a membrane protein localized in the Golgi apparatus.