Anti-KDEL Antibody
Mouse Monoclonal Antibody specific to KDEL Antibody
Product Specifications
Product Name Alternative
Anti-KDEL Antibody Mouse Monoclonal Antibody
Host
Mouse
Reactivity
All
Immunogen
Peptide corresponding to aa 649-654 of rat Grp78 conjugated to KLH.
Target
KDEL
Clonality
Monoclonal
Isotype
IgG2a
Type
Antibody
Applications
WB, ICC, ELISA
Field of Research
N
Purification Method
Purified by affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium which is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: Use at 4ug/ml. Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants.
<br><br>Immunohistochemistry: Use at 5- 10ug/ml.
<br><br>These are recommended concentrations.
<br><br>End users should determine optimal concentrations for their applications.
Storage Conditions
Stable for at least one year at or below -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Product Datasheet
https://www.qedbio.com/wp-content/uploads/pdf/11076.pdf
Specificity
KDEL Antibody
Formulation
PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.
Buffer pH
pH 7.2
Target Background
The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor which is a membrane protein localized in the Golgi apparatus.
CAS Number
9007-83-4
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