Recombinant Salmonella typhimurium Probable csgAB operon transcriptional regulatory protein (csgD)
Product Specifications
Abbreviation
Recombinant Salmonella typhimurium csgD protein
Gene Name
CsgD
UniProt
O54294
Expression Region
1-216aa
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Target Sequence
MFNEVHSSHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQRSLEDISAGCIVLMDMMEADKKLIHYWQDNLSRKNNNIKTLLLNTPDDYPYREIENWPHINGVFYATEDQEHVVSGLQGILRGECYFSQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR
Tag
N-terminal 6xHis-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
Necessary for transcription of the csgAB operon. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
28.9 kDa
References & Citations
"Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain." Wen Y., Ouyang Z., Devreese B., He W., Shao Y., Lu W., Zheng F. Protein Sci 26:2073-2082 (2017)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/12934514/
Protein Length
Full Length
Available Sizes
Curated Selection
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