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Recombinant Salmonella typhimurium Probable csgAB operon transcriptional regulatory protein (csgD)

Product Specifications

Abbreviation

Recombinant Salmonella typhimurium csgD protein

Gene Name

CsgD

UniProt

O54294

Expression Region

1-216aa

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Target Sequence

MFNEVHSSHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQRSLEDISAGCIVLMDMMEADKKLIHYWQDNLSRKNNNIKTLLLNTPDDYPYREIENWPHINGVFYATEDQEHVVSGLQGILRGECYFSQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Necessary for transcription of the csgAB operon. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

28.9 kDa

References & Citations

"Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain." Wen Y., Ouyang Z., Devreese B., He W., Shao Y., Lu W., Zheng F. Protein Sci 26:2073-2082 (2017)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

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