Anti-DNase II (CT) Antibody

Rabbit Polyclonal Antibody specific to Dnase II (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.1.22.1, Acid DNase, Deoxyribonuclease II α, DNase II α, Lysosomal DNase II, R31240_2

Gene Name

DNASE2

Gene ID

1777

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=DNASE2">DNASE2</a>

UniProt

O00115

Accession Number

NP_001366.1

Cellular Locus

Lysosome.

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 347- 360 of human DNase II precursor (accession no. AF047016).

Target Antigen

Deoxyribonuclease-2-α

Target

DNase II (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 5ugml. Positive control: Tissue lysate of human spleen. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes the proenzyme of human DNase II (40kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32kDa ( ) and12 kDa ( ) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.