Flubi-2
Flubi-2 is a fluorescein-biotin based pH-ratio dye (Ex=480 nm, Em= 520-560 nm) with a pK value of 6.7. Flubi-2 is a hydrolysis product of Flubida-2 (non-fluorescent, membrane permeable) . Flubi-2 can be used for pH determination of organelles of the secretory pathway (such as golgi apparatus and endoplasmic reticulum) in living cells[1][2].
Product Specifications
UNSPSC
12171500
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/flubi-2.html
Solubility
10 mM in DMSO
Smiles
O=C1C=CC(C(OC2=CC(O)=CC=C23)=C1)=C3C4=CC=C(C(N(C)CCCCCCN(C)C(CCCC[C@@]5([H])C(NC(N6)=O)C6CS5)=O)=O)C=C4C(O)=O.O=C7C=CC(C(OC8=CC(O)=CC=C89)=C7)=C9C%10=CC(C(N(C)CCCCCCN(C)C(CCCC[C@@]%11([H])C(NC(N%12)=O)C%12CS%11)=O)=O)=CC=C%10C(O)=O
Molecular Formula
C39H44N4O8S
Molecular Weight
728.85
References & Citations
[1]Wu MM, et al. Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates. Methods Enzymol. 2000;327:546-64. |[2]Wu MM, et al. Organelle pH studies using targeted avidin and fluorescein-biotin. Chem Biol. 2000 Mar;7 (3) :197-209.
Shipping Conditions
Room temperature
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Frequently Asked Questions
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