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Anti-Mouse IgG Fc specific Antibody, Alkaline Phosphatase conjugate

Goat Polyclonal Antibody specific to Mouse IgG Fc Fragment-Alkaline Phosphatase Conjugate

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Mouse IgG Fc Fragment-Alkaline Phosphatase Conjugate Goat Polyclonal Antibody

Host

Goat

Reactivity

Mouse

Immunogen

Mouse IgG-Fc fragment

Target

Mouse IgG Fc specific

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Secondary Antibodies

Purification Method

Purified by affinity chromatography on mouse IgA

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

HEPES

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000-1:10,000. <br><br>Immunohistochemistry: use at a dilution of 1:100-1:1,000 <br><br>Immunocytochemistry: use at a dilution of 1:100-1:1,000 <br><br>ELISA: use at a dilution of 1:5,000-1:50,000. <br><br>These are recommended dilutions. <br><br>End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.

Storage Conditions

This antibody is stable for at least 1 year at 2-8°C.

Specificity

By immunoblotting and ELISA this antibody reacts specifically with mouse IgG. Cross-reactivity with mouse IgA and IgM is negligible. This antibody may cross-react with IgG of other species.

Formulation

50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3

Buffer pH

pH 7.1

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