Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AS113) (Omicron Specific)

Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AS113) (Omicron Specific) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with omicron Spike RBD. The antibody is specific against the Omicron (B.1.1.529/BA.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus and other viral lineages.

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

CAS Number

9007-83-4

Host

HEK293

Origin Species

Human

Target

Spike RBD

Conjugation

Unconjugated

Tag

Human IgG1 | Human Kappa

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

Purity

0.95

Bioactivity

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No.SPD-C522e) and SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) and SARS-CoV-2 Spike RBD, His Tag (BA.1+L452R/Omicron) (Cat. No. SPD-C522k) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M415) with a linear range of 0.049-3.125 ng/mL. The antibody does not bind SARS-CoV-2 Spike RBD WT (Cat. No. SPD-C52H3), Delta (Cat. No. SPD-C52Hh), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr), Alpha (Cat. No. SPD-C52Hn) (Routinely tested).