DNA Polymerase I (10 U/μL)

DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'—3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'—3' exonuclease activity and single-strand-specific 3'—5 exonuclease activity. This enzyme can be used with DNase I (Cat#10607) for nick translation reaction and with RNase H (Cat#12909) to synthesize the second strand of cDNA.