Anti-Estrogen Receptor α Antibody

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). {PubMed:10681512, PubMed:10816575, PubMed:11477071, PubMed:11682626, PubMed:14764652, PubMed:15078875, PubMed:15891768, PubMed:16043358, PubMed:16617102, PubMed:16684779, PubMed:17922032, PubMed:17932106, PubMed:18247370, PubMed:19350539, PubMed:20074560, PubMed:20705611, PubMed:21330404, PubMed:22083956, PubMed:7651415, PubMed:9328340}.; [Isoform 3]: Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861). {PubMed:10970861, PubMed:21937726}.

Immunoblotting: use at 1-5ug/mL. <br><br>Immunohistochemistry: frozen sections, use at 1-10ug/mL.FACS: use at 1-10ug/mL. <br><br>Immunoprecipitation: use at 1-5ug/mL. <br><br>Positive controls: MCF-7 cells and recombinant protein. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

This antibody recognizes human and rabbit ER-alpha, a 65 kD protein and a member of the steroid family of nuclear receptors. ER-alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, ER-alpha can positively or negatively regulate gene transcription.