CD8 Mouse Monoclonal Antibody (PE)
The CD8 Antibody-PE labeled is suitable for FC, IF. It is a Monoclonal, PE conjugated antibody which raised against Native purified human CD8.. Purification: The antibody was purified by affinity chromatography.
Product Specifications
Product Name Alternative
MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a; CD8B1; T-cell surface glycoprotein CD8 beta chain; CD antigen CD8b
UniProt
P01732, P10966
Reactivity
Human
Immunogen
Native purified human CD8.
Target
CD8A; CD8B
Clonality
Monoclonal
Clone
BTKX33
Conjugation
PE
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Dilution
10 μl / assay
Form
Liquid
Storage Conditions
Shipped and store at 4°C for one year. Do not freeze.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Isotype
Mouse IgG1
Entrez
926, 925
Available Sizes
Frequently Asked Questions
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