Ly-49C/F/H/I Golden Hamster Monoclonal Antibody (PE)

Ly-49c; Ly49C; Killer cell lectin-like receptor 3; 5E6; Lymphocyte antigen 49c; Ly-49c; Nk2.1; T-cell surface glycoprotein Ly-49C; Ly-49f; Ly49-f; Ly49F; Killer cell lectin-like receptor 6; Lymphocyte antigen 49f; Ly-49f; T-cell surface glycoprotein Ly-49F; Ly-49h; Ly49-h; Ly49H; Killer cell lectin-like receptor 8; Lymphocyte antigen 49h; Ly-49h; T-cell surface glycoprotein Ly-49H; Killer cell lectin-like receptor subfamily A member 9; Killer cell lectin-like receptor subfamily A member 9; Natural killer cell recptor Ly49C

1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .