CD8a Mouse Monoclonal Antibody

The CD8a Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD8a .Purification: The antibody was purified by affinity chromatography.

Product Specifications

Product Name Alternative

MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a

UniProt

P01732

Reactivity

Human

Immunogen

Native purified human CD8a

Target

CD8A

Clonality

Monoclonal

Clone

BJKV0C

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

The antibody was purified by affinity chromatography.

Dilution

IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .