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HLA-A/B/C Mouse Monoclonal Antibody

The HLA-A/B/C Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody .Purification: The antibody was purified by affinity chromatography.

Product Specifications

Product Name Alternative

HLA-A; HLAA; HLA class I histocompatibility antigen, A-68 alpha chain; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68; HLA-A; HLAA; HLA class I histocompatibility antigen, A-2 alpha chain; MHC class I antigen A*2; HLA-A; HLAA; HLA class I histocompatibility antigen, A-3 alpha chain; MHC class I antigen A*3; HLA-A; HLAA; HLA class I histocompatibility antigen, A-24 alpha chain; Aw-24; HLA class I histocompatibility antigen, A-9 alpha chain; MHC class I antigen A*24; HLA-A; HLAA; HLA class I histocompatibility antigen, A-32 alpha chain; MHC class I antigen A*32; HLA-A; HLAA; HLA class I histocompatibility antigen, A-69 alpha chain; Aw-69; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*69; HLA-A; HLAA; HLA class I histocompatibility antigen, A-11 alpha chain; MHC class I antigen A*11; HLA-A; HLAA; HLA class I histocompatibility antigen, A-31 alpha chain; MHC class I antigen A*31; HLA-A; HLAA; HLA class I histocompatibility antigen, A-25 alpha chain; HLA class I histocompatibility antigen, A-10 alpha chain; MHC class I antigen A*25; HLA-A; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain; MHC class I antigen A*1; HLA-A; HLAA; HLA class I histocompatibility antigen, A-26 alpha chain; MHC class I antigen A*26; HLA-A; HLAA; HLA class I histocompatibility antigen, A-34 alpha chain; Aw-34; HLA class I histocompatibility antigen, A-10 alpha chain; MHC class I antigen A*34; HLA-A; HLAA; HLA class I histocompatibility antigen, A-36 alpha chain; Aw-36; MHC class I antigen A*36

UniProt

P04439, P10321, P01889

Reactivity

Human

Target

HLA-A; HLA-B; HLA-C

Clonality

Monoclonal

Clone

BY8354

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

The antibody was purified by affinity chromatography.

Dilution

IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .

Tested Applications

FC, IF

Host or Source

Mouse

Preservative

PBS, pH 7.3, and 0.02% sodium azide.

Isotype

Mouse IgG2a kappa

Entrez

3105

Available Sizes

Frequently Asked Questions

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