Anti-Chromogranin B Antibody

Rabbit Polyclonal Antibody specific to Chromogranin B

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Chromogranin-B, CgB, Secretogranin I, SgI [Cleaved into: PE-11; GAWK peptide; CCB peptide]

Gene Name

CHGB

NCBI Gene ID

UniProt

P05060

Cellular Locus

Secreted. Note=Neuroendocrine and endocrine secretory granules.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from the C-terminus (aa 641-655) of CgB (accession no. NP_001810. This sequence is identical in human, mouse, and rat CgB.

Target Antigen

Secretogranin-1

Target

Chromogranin B

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Hormones/Proteins

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.

Additionnal Information

Immunoblotting: use at 0.1-1.0ug/mL These are recommended concentrations. Enduser should determine optimal concentrations for their applications.Although the predicted mw of CgB is ~76kDa, the highly acidic nature of CgB causes it to run at a higher mw in SDS-PAGE. Immunohistochemistry: use at 1-5ug/mL.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat chromogranin B.

Formulation

PBS, pH 7.4

Buffer pH

7.4

Target Background

Chromogranin B (CgB, secretogranin I) is a secretory granule matrix protein expressed in a wide variety of endocrine cells and neurons where it may have both extracellular and intracellular functions. Extracellular roles for CgB are dependent on its proteolytic processing within secretory granules to form smaller peptides that may have a role in the neuroendocrine/sympathoadrenal stress response to systemic infection, and CgB fragments may also have an autocrine inhibitory role in the release of co-stored hormones, such as insulin.