ConA-Biotin Conjugated
Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins.
Product Specifications
Applications
WB, ELISA
Assay Principle
Histochemistry 1: 250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500.
Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions.
For Histochemistry the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied followed by streptavidin conjugated to enzyme and chromogen. For WB incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen. For ELISA the target proteins are absorbed on ELISA plate at a concentration of 5-10 µg/ml, followed by blocking with protein solution followed by Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate. Please refer to Histochemistry, WB and ELISA protocol for detail information.
Shipping Conditions
Blue Ice
Storage Conditions
4°C
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