Erythromycin ELISA Kit

Erythromycin is a broad-spectrum antibiotic that belongs to the class of macrolides that contains Azithromycin, Spiramycin, and others. This bacteriostatic drug is produced by Saccharopolyspora erythraea and is effective against both gram-positive and gram-negative bacteria. The mechanism of action is that erythromycin binds to 23S rRNA of the 50S ribosomal subunit. In doing so, it prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. Antimicrobials such as Erythromycin are extensively used in food-producing animals to treat infectious diseases. According to International Food Standards, Maximum Residue Limits (MRLs) for erythromycin for tissues of food-producing species (such as chicken and turkey) is 100 μg/kg (ppb) and in eggs is 50 μg/kg (ppb). BioVision’s Erythromycin ELISA kit is used to quantitatively measure Erythromycin in tissue and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Erythromycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Erythromycin in the samples.