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Anti-Hu CD369 APC

CD369 (dectin-1, beta-glucan receptor) is a 33 kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Extracellular part of human CD369 with hIgG FC tag

Target Antigen

CD369

Clone

15E2

Applications

FC (QC tested)

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Ni L, Gayet I, Zurawski S, Duluc D, Flamar AL, Li XH, O'Bar A, Clayton S, Palucka AK, G Zurawski, Banchereau J, Oh SK: Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010 Sep 15;185 (6) :3504-13, URL: https://pubmed.ncbi.nlm.nih.gov/20729328/?from_term=ni+l+gayet+i+zurawski+s&from_pos=2, *Fischer M, Müller JP, Spies-Weisshart B, Gräfe C, Kurzai O, Hünniger K, Hochhaus A, Scholl S, Schnetzke U. Isoform localization of dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 2017 May;47 (5) :848-859 (FC), URL: https://pubmed.ncbi.nlm.nih.gov/28303575/?from_term=scholl+s+hochhaus+a&from_pos=5, *Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li XH, Hori T, Zurawski S, Liu YJ, Zurawski G, Oh S: Opposing roles of dectin-1 expressed on human plasmacytoid dendritic cells and myeloid dendritic cells in Th2 polarization. J Immunol. 2015 Aug 15;195 (4) :1723-31, URL: https://pubmed.ncbi.nlm.nih.gov/26123355/?from_term=zhang+w+xue+y&from_page=6&from_pos=10

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.

Applications Notes

6

Isotype

Mouse IgG2a kappa

Frequently Asked Questions

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