Anti-DRAK1 (NT) Antibody

Rabbit Polyclonal Antibody specific to DRAK1 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.11.1, DAP kinase-related apoptosis-inducing protein kinase 1

Gene Name

STK17A

Gene ID

9263

NCBI Gene ID

UniProt

Q9UEE5

Accession Number

NP_004751.2

Cellular Locus

Nucleus

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 5-19 of human DRAK1 (accession no. Q9UEE5).

Target Antigen

Serine/threonine-protein kinase 17A

Target

DRAK1 (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Acts as a positive regulator of apoptosis. Also acts as a regulator of cellular reactive oxygen species. {PubMed:21489989, PubMed:9786912}.

Additionnal Information

Immunoblotting : use at 1ug/mL. Immunocytochemistry: use at 2ug/mL. Positive control: Whole cell lysate from A431 or MOLT4 cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human DRAK1 (50kDa). No cross-reactivity with DRAK2, DAP or ZIP kinases.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis have been identified and designated DRAK1 and DRAK2 for DAP kinaseâ€“related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C- terminal regulation domain. Overexpression of DRAK1 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases which mediate apoptosis through their catalytic activities. DRAK1 is located in cell nuclei, and the mRNA for DRAK1 is ubiquitously expressed in human tissues.