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Anti-Hu IgG (Fab) Biotin

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.

Product Specifications

Certification

RUO

Immunogen

Purified human IgG.

Target Antigen

IgG (Fab)

Clone

4A11

Applications

FC (QC tested), WB, ELISA

Concentration

1 mg/mL

Format

Biotin

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152 (2) :323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141?ordina, *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39 (2) :129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908?ordina, *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21 (6) :667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610?ordina, *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.

Applications Notes

Flow cytometry: Recommended dilution: 0.5-4 μg/ml.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

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