(±) -trans-1,2-Bis (2-mercaptoacetamido) cyclohexane

(±) -trans-1,2-Bis (2-mercaptoacetamido) cyclohexane is a small-molecule dithiol catalyst with a low thiol pKa value (8.3) and high reduction potential (-0.24 V), capable of mimicking PDI activity. It catalyzes the activation of scrambled ribonuclease A (scrambled ribonuclease A) and promotes the formation of native disulfide bonds, thereby significantly enhancing protein folding efficiency. Adding (±) -trans-1,2-Bis (2-mercaptoacetamido) cyclohexane to the culture medium of Saccharomyces cerevisiae can increase the secretion of exogenously expressed Schizosaccharomyces pombe acid phosphatase by more than threefold. (±) -trans-1,2-Bis (2-mercaptoacetamido) cyclohexane holds great potential for applications in protein production and secretion research[1].