ANS

ANS (8-Anilino-1-naphthalenesulfonic acid) is a competitive inhibitor targeting thyroxine binding globulin (TBG) (Ki=2.09x106 M-1) . ANS is used in radioimmunoassay by displacing bound triiodothyronine (T3) to improve detection sensitivity. ANS can block the protein binding site of T3 and release free T3 for antibody recognition. As a fluorescent probe, ANS can specifically bind to the hydrophobic region of proteins (such as membrane proteins) and monitor the dynamics of protein conformation through changes in fluorescent signals. It is widely used in biochemical research and antibacterial material development[1][2].

Product Specifications

CAS Number

[82-76-8]

Product Name Alternative

ANSA; 8-Anilino-1-naphthalenesulfonic acid

UNSPSC

12352200

Hazard Statement

H315, H319

Target

Bacterial

Type

Biochemical Assay Reagents

Related Pathways

Anti-infection

Field of Research

Infection

Assay Protocol

https://www.medchemexpress.com/ans.html

Purity

98.01

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

O=S(O)(C1=CC=CC2=CC=CC(NC3=CC=CC=C3)=C21)=O

Molecular Formula

C16H13NO3S

Molecular Weight

299.35

Precautions

H315, H319

References & Citations

[1]Polak J, et al. Bioactive Properties of a Novel Antibacterial Dye Obtained from Laccase-Mediated Oxidation of 8-Anilino-1-naphthalenesulfonic Acid. Molecules. 2022 Jan 13;27 (2) :487.|[2]Brown ML, et al. Use of 8-anilino-1-naphthalenesulfonic acid in radioimmunoassay of triiodothyronine. J Pharm Sci. 1974 Aug;63 (8) :1214-7.