Glycolic Acid Assay Kit (Fluorometric)

Glycolic Acid (GA), also known as Hydroxyl Acetic Acid is the smallest alpha hydroxy acid. Although it is used in several skin care products intended for dermal application in small doses, its acute dose can lead to skin and eye irritation. It is nephrotoxic if swallowed and its toxicity is due to its conversion to oxalic acid, which then precipitates with calcium to form crystals leading to renal tissue injury. GA is also a major downstream metabolite of ethylene glycol metabolism, and a concentration higher than 8 mM in serum indicates the need for hemodialysis. Normally serum GA levels lie in the very low micromolar range (roughly 2 µM). BioVision’s Glycolic Acid Assay Kit is a robust, one step plate based assay for the measurement of GA in serum, urine and plasma samples. The GA Detection Reagent metabolizes GA leading to the formation of an intermediate. The intermediate in the presence of GA enzyme mix then converts the non-fluorescent GA probe to a fluorescent product, which is measured at Ex/Em = 535/587 nm. This kit can quantify as low as 30 pmol of GA.