Anti-Hsp40 (Ydj1) Antibody

Mouse Monoclonal Antibody specific to Hsp40 (Ydj1)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Yeast dnaJ protein 1

Gene Name

YDJ1

Gene ID

855661

NCBI Gene ID

UniProt

P25491

Accession Number

NP_014335.1

Cellular Locus

Cytoplasm. Cytoplasm, perinuclear region. Note=Concentrated in a perinuclear ring as well as in the cytoplasm.

Host

Mouse

Reactivity

Yeast

Immunogen

Full-length yeast Hsp40 (Ydj1)

Target Antigen

Mitochondrial protein import protein MAS5

Target

Hsp40 (Ydj1)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, IP

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex. {PubMed:11689685}.

Additionnal Information

Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected <br><br>User should should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes yeast Hsp40 (Ydj1). It does not cross-react with human, mouse, or rat Hsp40.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins.