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S100B Antibody

S100B Antibody

Product Specifications

Product Name Alternative

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

UniProt

P04271

Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

Target

S100B

Clonality

Recombinant

Clone

S100B/1706R

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein A affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLWestern blot: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.25-0.5 μg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1) The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG, kappa

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