DR6 Antibody [Luke-1]

DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1) . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line) . Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.