Anti-GATA1 (phospho-Ser142) Antibody

Rabbit Polyclonal Antibody specific to GATA1 (phospho-Ser142)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-GATA1 (phospho-Ser142) Rabbit Polyclonal Antibody

Gene Name

GATA1

Gene ID

305371

NCBI Gene ID

UniProt

P15976

Cellular Locus

Nucleus.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 142 (R-L-S(p)-P-D) derived from human GATA1 and conjugated to KLH.

Target Antigen

Erythroid transcription factor

Target

GATA1 (phospho-Ser142)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). {PubMed:22235304, PubMed:24245781}.

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat GATA1 only when phosphorylated at serine 142.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

GATA1 encodes a protein, GATA1, which belongs to the GATA family of transcription factors. This protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.