Mouse anti Human LEF-1 transactivation domain

Product Specifications

Background

Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.

CAS Number

9007-83-4

Synonyms

Anti-Lymphoid Enhancer Factor-1, Transactivation Domain (amino acids 236-242) Monoclonal Antibody

UniProt

Q9UJU2

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Clone

3A12

Conjugation

Unconjugated

Type

Monoclonal Antibody

Source

Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of Human LEF-1 and mouse myeloma cells

Applications

Field of Research

Signal Transduction

Purification Method

Protein A/G Chromatography

Assay Principle

Antibody detects amino acids 236-242 of Human LEF-1. Epitope is missing in some splice variants. Optimal concentration should be evaluated by serial dilutions.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Riese, J., et al., LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic. Cell 1997, 88, 777-787_x000D_ 2. Giese, K., et al. Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1. Proc. Natl. Acad. Sci. USA 1997, 94, 12845-12850_x000D_ 3. Behrens, J., et al. Functional interaction of beta-catenin with the transcription factor LEF-1. Nature 1996, 382, 638-642_x000D_ 4. Giese, K. and Grosschedl, R. LEF-1 contains an activation domain that stimulates transcription only in a specific context of factor-binding sites. EMBO J. 1993, 12, 4667-4676